PCR amplification of ribosomal DNA for species identification in the plant pathogen genus Phytophthora
Article Abstract:
A polymerase chain reaction method for DNA amplification and the quick identification of important plant-pathogenic Phytophthora species from the six taxonomic groups of the genus was designed. The ITS primers 5 and 4 and PCR were used to amplify the entire rDNA gene, both ITS regions I and II and a part of the 18S nuclear small-subunit rDNA gene. A series of restriction enzymes were then utilized to cut the amplified DNA to develop species-specific restriction fragment patterns. The PCR techniques can be potent tools for identifying the major species in the genus Phytophthora.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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High-resolution genotyping of Campylobacter strains isolated from poultry and humans with amplified fragment length polymorphism fingerprinting
Article Abstract:
A genotyping method using amplified fragment length polymorphism (AFLP) was developed for epidemiological studies of Campylobacter jejuni and Campylobacter coli infections in humans and poultry. This method was based on selective amplification of restriction fragments of chromosomal DNA. The AFLP fingerprinting method uses restriction endonucleases HindIII and HhaI in combination with one set of selective polymerase chain reaction primers. The band patterns of 10 C. jejuni strains isolated from humans were heterogeneous, and most of these strains grouped with poultry strains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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