PCR amplification of the fimA gene sequence of Salmonella typhimurium, a specific method for detection of Salmonella spp
Article Abstract:
Primers consisting of parts of the fimA gene sequence are used for the polymerase chain reaction (PCR) amplification and detection of Salmonella strains. The fimA gene sequences, encoding the major fimbrial unit, are unique to Salmonella strains. The utilization of the fimA gene as a probe to differentiate between Salmonella and non-Salmonella strains provides a valuable diagnostic tool. The PCR method is effective in detecting Salmonella strains from contaminated samples of food and milk.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Development and application of pathovar-specific monoclonal antibodies that recognize the lipopolysaccharide O antigen and the type IV fimbriae of Xanthomonas hyancinthi
Article Abstract:
Research was conducted to create a specific immunological diagnostic assay for yellow disease in hyacinths using monoclonal antibodies (MAbs). The MAbs were obtained by immunizing mice with ppurified fimbriae and shear fractions of X hyacinth. It was observed that antifimbria MAbs reacted with various fimbrial epitopes. The cross-reaction of the MAbs with all strains of X hyacinthi tested revealed that the Rha-Fuc O antigen is conserved within the species.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Analysis of the type IV fimbrial-subunit gene fimA of Xanthomonas hyacinthi: application in PCR-mediated detection of yellow disease in hyacinths
Article Abstract:
A PCR-based technique for detecting Xanthomonas hyacinthi infection of hyacinths is described. This pathogen causes yellow disease of hyacinths and other members of the Liliaceae family.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Alteration of the rugose phenotype in waaG and ddhC mutants of Salmonella enterica serovar Typhimurium DT104 is associated with inverse production of curli and cellulose
- Abstracts: Functional analysis of the flagellar genes in the fliD operon of Salmonella typhimurium. The Salmonella FigA protein, a putative periplasmic chaperone essential for flagellar P ring formation
- Abstracts: Differential cytokine expression in avian cells in response to invasion by Salmonella typhimurium, Salmonella enteritidis and Salmonella gallinarum
- Abstracts: Regulation of the ndh gene of Escherichia coli by integration host factor and a novel regulator, Arr. Enhanced production of insulin-like growth factor I fusion protein in Escherichia coli by coexpression of the down-regulated genes identified by transcriptome profiling
- Abstracts: Immunoaffinity concentration and purification of waterborne enteric viruses for detection by reverse transcriptase PCR