PCR-based genotyping of epidemic and preepidemic Trichoderma isolates associated with green mold of Agaricus bisporus
Article Abstract:
The biotype 4 strain of Trichoderma atroviride (T. atroviride) was the prominent cause of the green mold epidemic episodes which plagued the mushroom-growing region of North America during the 1950s. Through the use of randomly amplified polymorphic DNA (RAPD)-PCR, it was established that T. atroviride biotype 4 caused 90% of the green mold epidemic episodes during the period mentioned. However, results nullified the existence of preepidemic isolates in the region, possibly due to the fact that majority of the isolates have been isolated from compost and wooden surfaces.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Characteristics of a hydrated, alginate-based delivery system for cultivation of the button mushroom
Article Abstract:
Solid support matrices, such as calcium alginate gel, can be used as a delivery system for soil-borne biocontrol fungi. The possibility of using this technology in cultivating button mushroom was investigated, leading to a novel technique for mushroom cultivation. Agaricus bisporus was allowed to colonize alginate-based pellets and disseminated in a hydrated condition. The application of this technique resulted in the production of mushrooms with similar size and yields as those obtained from composts seeded with other forms of carriers.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Growth of Mycoplasma hyorhinis cultivar alpha on semisynthetic medium
Article Abstract:
The growth of the Mycoplasma hyorhinis cultivar alpha strains in liquid modified CMRL-1066 medium containing fetal bovine serum is similar to that of the other strains. In medium containing bovine albumin, palmitic acid and cholesterol, the growth is slow and the number of cells is less as compared to the growth in medium containing serum. Thus the reason the cultivar alpha strains do not grow in the medium usually used is that their growth is stopped by the inhibiting-factors of some of the components of the medium .
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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