Plasmids of the pRM/pRF family occur in diverse Rickettsia species
Article Abstract:
The existence of plasmids in Rickettsia helvetica, R. peacockii, R. amblyommii and R.massiliae isolates from ixodid ticks and in an R. hoogstraalii isolate from an argasid tick is described. The occurrence of plasmids in a diverse range of Rickettsia spp. has shown that they are widespread in the genus and their existence in the face of pressure exerted by reductive genome evolution has shown a crucial role in rickettsial biology.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2008
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Transposon insertion reveals pRM, a plasmid of Rickettsia monacensis
Article Abstract:
A plasmid, pRM from Rickettsia monacensis which was detected by pulsed-field gel electrophoresis and Southern blot analyses of DNA transformed by transposon-mediated insertion is described. It was seen that pRM was present in rickettsial cells as circular and linear isomers and based on BLAST searches of the GenBank database, pRM contained 23 predicted genes or pseudogenes and was similar to the larger pRF plasmid.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Analysis of fluorescent protein expression in transformants of Rickettsia monacensis, an obligate intracellular tick symbiont
Article Abstract:
Transposon for simultaneous expression of a reported gene and a chloramphenicol acetyltransferase (CAT) resistance marker is designed using the Epicentre EZ::TN transposon system. Transposon-based vectors are developed and applied for molecular manipulation and analysis of spotted fever group rickettsiae, which are obligate intracellular bacteria that infect ticks, and, in some cases, mammals.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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