Dynamic organization of chromosomal DNA in Escherichia coli
Article Abstract:
Dynamic organization of chromosomal DNA in Escherichia coli, organization critical in cell division, chromosome replication, and partitioning in the bacterial cell cycle, is discussed. The subcellular localization of 22 DNA segments of the E. coli chromosome has been studied by the FISH method. The bacterial chromosome is 1000 times the length of the bacterium and folded by some unknown means into a nucleoid, a compact form. The circular chromosomal DNA has been shown to be organized to form a compact ring structure in the cell.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2000
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Subcellular distribution of actively partitioning F plasmid during the cell division cycle in E. coli
Article Abstract:
F plasmid, the fertility factor of E. coli, is partitioned with fidelity to daughter cells during cell division, due to sopA and sopB, trans-acting genes, and sopC, a cis-acting site. The subcellular distribution of mini-F-plasmid molecules was visualized through fluorescence in situ hyrbidization. Molecules lacking the sopABC segment showed random distribution in spaces not occupied by nucleoids.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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Polar localization of the replication origin and terminus in Escherichia coli nucleoids during chromosome partitioning
Article Abstract:
Three migration patterns are discernible in the replication origin (oriC), the chromosome terminus and other DNA regions during the partitioning of daughter chromosomes in Escherichia coli. In cell division cycle by FISH, oriC is at the old-pole-proximal and the terminus is at the new-pole-proximal nucleoid borders. OriC, which travel to opposite nucleoid borders, are retained and stay a constant distance from each cell pole.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
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