Polymerase chain reaction and gene probe detection of the 2,4-dichlorophenoxyacetic acid degradation plasmid, pJP4
Article Abstract:
Use of polymerase chain reaction and gene probe detection increases detection of biodegradative organisms. Optimal use of biodegrading microorganisms require their identification in environmental samples. Genes encoding for such capabilities are often located in bacterial plasmids. Studies show that polymerase chain reaction (PCR) can amplify the 2,4-dichlorophenoacetic acid degradation plasmid pJP4 in Alcaligenes eutrophus to enable more sensitive detection by gene probes. This combined use of PCR gene probe detection provides a more sensitive method of microbial detection than traditional cultural methods.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Specific detection of Salmonella spp. by multiplex polymerase chain reaction
Article Abstract:
Multiplex polymerase chain reaction amplification with three sets of primers, phoP, Hin and H-li, was used for specific detection of Salmonella species. The phoP primer has the ability to detect coliform pathogenic bacteria such as Salmonella, Shigella, Escherichia coli and Citrobacter spp. Hin and H-li primers are specific to motile Salmonella spp. and are not present in E. coli. It was thus demonstrated that this analysis is sensitive and allows detection of Salmonella spp. in environmental samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Detection of enteroviruses in groundwater with the polymerase chain reaction
Article Abstract:
The detection of enteroviruses by polymerase chain reaction (PCR) was described. The RNA-PCR assay was used to evaluate the effectiveness of Sephadex G-50, Sephadex G-100, Sephadex G-200, Chelex-100 resin and a mixed bed resin in removing inhibitory material from groundwater concentrates and humic acid solutions. It was found that Sephadex G-100 plus Chelex-100 is very effective in removing inhibitory substances for the detection of enteroviruses in these samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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