Preparation of refractile spores of Clostridium thermosaccharolyticum involves a solventogenic phase
Article Abstract:
Culture conditions were developed to convert vegetative cells of Clostridium thermosaccharolyticum to refractile endospores.The procedure involved sequential dilution into fresh medium at each generation with a final dilution into a xylan sporulation medium containing excess calcium. Under these conditions, a total concentration of 9.63% of butanol, ethanol and isopropanol was achieved by synchronized, elongated and solventogenic cells of the clostridia. Solventogenesis continued until the cells entered the spore phase. The solventogenic phase appeared to be an essential step for sporulation.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Involvement of an intracellular oligogalacturonate hydrolase in metabolism of pectin by Clostridium thermosaccharolyticum
Article Abstract:
The metabolic effect of Clostridium thermosaccharolyticum to oligogalacturonates and the enzymatic synthesis involved in the regulation of pectin degradation of this organism were discussed. Experiments involved transport studies, gel filtration chromatography and molecular weight estimation of the oligogalacturonase. Induction amd catabolite repression were found to control the clostridium organism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Isolation and characterization of an extracellular glycosylated protein complex from Clostridium thermosaccharolyticum with pectin methylesterase and polygalacturonate hydrolase activity
Article Abstract:
A comparison of the features of the pectinolytic enzymes from a pectin thermophilic organism and those from Clostridium thermosaccharolyticum is discussed. Experiments involved molecular weight estimation, electrophoresis, enzyme purification, monosaccharide analysis, amino acid analysis and N-terminal sequence analysis. Differences in the pectinolytic systems of both bacterium was demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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