Molecular study and overexpression of the Clostridium cellulolyticum celF cellulase gene in Escherichia coli
Article Abstract:
The cloning, sequencing and expression of the celF gene of Clostridium cellulolyticum corresponding to the open reading frame-1 in the cel gene cluster are discussed. Expression of celF in Escherichia coli under standard conditions caused the recombinant protein to aggregate and form inclusion bodies. A mature form of CelF was generated after optimizing the induction conditions. CelF hydrolyzed cellulose but was poorly active on substituted cellulose.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
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Gene transfer to Clostridium cellulolyticum ATCC 35319
Article Abstract:
Research has been conducted on bacterial cellusome and its protein components. The study of their role in bacterial growth on cellulose has been carried out via the use of gene transfer techniques developed for Clostridium cellulolyticum ATCC 35319, and the details are reported.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
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YeiL, the third member of the CRP-FNR family in Escherichia coli
Article Abstract:
Research has been conducted on YeiL protein from Escherichia coli. The characterization of this protein has been carried out, and its functions have been defined via the study of the physiological consequences of yeiL gene inactivation and yeiL gene expression regulation.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2000
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- Abstracts: Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-alpha-decarboxylase leads to pantothenate overproduction in Escherichia coli
- Abstracts: Recruitment and expression of toluene/trichloroethylene biodegradation genes in bacteria native to deep-subsurface sediments
- Abstracts: Zeocin resistance suppresses mutation in hypermutable Escherichia coli. Mutations in the beta subunit of the Bacillus subtilis RNA polymerase that confer both rifampicin resistance and hypersensitivity to NusG
- Abstracts: Antiterminator protein GlpP of Bacillus subtilis binds to glpD leader mRNA. The aprE leader is a determinant of extreme mRNA stability in Bacillus subtilis