Procaryotic expression of single-chain variable-fragment (scFV) antibodies: secretion in L-form cells of Proteus mirabilis leads to active product and overcomes the limitations of periplasmic expression in Escherichia coli
Article Abstract:
A study was conducted to analyze the expression of various antibody constructs in Proteus mirabilis L Vi and compare it with their expression in Escherichia coli JM109. DNA manipulations were carried out using conventional techniques while the E. coli K-12 strain JM109 was utilized as the cloning and expression host. Results indicated that the expression of the antibody constructs in L-form cells were superior than their expression in E. coli based on product yield.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Morphological and physiological changes induced by high hydrostatic pressure in exponential- and stationary- phase cells of Escherichia coli: relationship with cell death
Article Abstract:
The various morphological and physiological changes that occur in pressurized Escherichia coli cells that might correlate with cell death are examined. The study of changes in gross morphology, loss of membrane integrity, the loss of osmotic responsiveness, changes in the conformation of nucleic acids, and protein coagulation in cells is presented.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Characterization of chloroethylene dehalogenation by cell extracts of Desulfomonile tiedjei and its relationship to chlorobenzoate dehalogenation
- Abstracts: Proteinaceous factor(s) in culture supernatant fluids of bifidobacteria which prevents the binding of enterotoxigenic Escherichia coli to gangliotetraosylceramide
- Abstracts: Serologic and molecular characterization of Vibrio parahaemolyticus strains isolated from seawater and fish products of the Gulf of Mexico
- Abstracts: Contribution of anaerobic protozoa and methanogens to hindgut metabolic activities of the American cockroach, Periplaneta americana
- Abstracts: Comparison of different procedures for stereotyping aquatic birnavirus. Survival of fecal coliforms in dry-composing toilets