Production and characterization of Trametes versicolor mutants unable to bleach hardwood kraft pulp
Article Abstract:
A mutant form of the fungus Trametes versicolor that does not produce manganese peroxidase (MnP) appears unable to delignify and bleach kraft pulp and has reduced ability to oxidize dyes. Researchers isolated the M49 mutant strain of the white rot fungus and found that, in comparison to a wild-type strain, it produced less laccase, ehthylene, and methanol when exposed to different substrates. When MnP was added to the culture some of the normal fungal activity was restored.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Manganese peroxidase, produced by Trametes versicolor during pulp bleaching,demethylates and delignifies kraft pulp
Article Abstract:
The production of laccase and manganese peroxidase (MnP) by Trametes versicolor during pulp bleaching is discussed. The fungal bleaching rate coincided with the level of secreted MnP. Ion-exchange chromatography was used to separate laccase and MnP. A preference for the oxidation of phenolic lignin structures was shown to be preferred by MnP on the basis of methanol release and the methoxyl contents of the pulp.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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Lignin oxidation by laccase isozymes from Trametes versicolor and role of the mediator 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) in kraft lignin depolymerization
Article Abstract:
The fungus Trametes versicolor produces two laccase isoenzymes (I and II) which polymerize wood and pulp lignin. If the substrate has a low molecular weight the delignification activity of these isoenzymes is similar, but if polymeric substrates are used laccase I is more active. 2,2'-azinobis(3-ethylbenzthiazoline-6-sulfonate) inhibits the delignification of kraft wood and pulp and induces relignification .
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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