Production of an extracellular polyethylene-degrading enzyme(s) by Streptomyces species

Article Abstract:

Streptomyces viridosporus T7A, Streptomyces badius 252 and Streptomyces setonii 75Vi2 produce extracellular enzyme(s) that are capable of degrading heat-treated polyethylene degradable plastics. A preliminary study showed that enzyme(s) produced by these Streptomyces species enabled changes in tensile strength, percent elongation and strain energy of these plastic films during incubation. This was determinedby an Instron model 4502 Universal Tester. Also, reduction in polyethylene molecular weight distribution was observed during incubation with the active enzymes as determined by high-temperature gel permeation high-pressure liquid chromatography. Increased infrared spectrum was also observed in active-enzyme treated plastic films as determined by the Fourier transform infrared spectrometer.

Plastics, Streptomyces, Polyethylene, Biodegradable plastics

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Characterization of an extracellular protease inhibitor of Bacillus brevis HPD31 and nucleotide sequence of the corresponding gene

Article Abstract:

An extracellular protease inhibitor(PI) from Bacillus brevis was isolated and characterized. This PI, designated BbrPI, was found to be in multiple forms with the same precursor molecule as demonstrated by Western blot analysis. DNA sequence analysis of the gene encoding BbrPI showed that the amino acid sequence from one to twentyfour have characteristicstypical of signal peptides of PI precursors. These precursors were presumed to be cleaved by the enzyme protease into active PIs. Characterization studies of these inhibitors are however, limited. This study was able to establish the distinction of BbrPI from other PIs based on the absence of cysteine residues in its molecule but further studies to determine its role in Bacillus brevis was not done due to failure in disrupting its gene.

Genetic aspects, Protease inhibitors, Bacterial proteins, Enzyme inhibitors

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Nucleotide sequence and replication properties of the Bacillus borstelensis cryptic plasmid pHT926

Article Abstract:

The cryptic plasmid pHT926 from Bacillus borstelensis was sequencedand characterized. The plasmid, the first from the B. brevis group to be completelysequenced, was found to belong to the pC194 plasmid family. It appears to replicate bythe rolling-circle mechanism. Its copy number, which is four times that of pUB110, andits stability in B. choshinensis suggest that it could be used to make expression- secretionvectors for foreign proteins.

Analysis, Usage, Plasmids, Nucleotide sequence, Base sequence, Mutation (Biology), Mutation, Protein engineering

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