Production of poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) in a recombinantEscherichia coli strain
Article Abstract:
Incorporation of poly-3-hydroxybutyrate (PHB) genes from Alcaligenes eutrophus into an Escherichia coli fadR atoC (Con) mutant produces an E. coli strain that is capable of producing the copolymer poly-(3-hydroxybutyrate-co-3-hydroxyvalerate) (P(HB-co-HV)). This copolymer haswide industrial use and is made by the incorporation of 3-hydroxyvalerate into the homopolymer poly-3-hydroxybutyrate. This incorporation is greatly dependenton the presence of propionate and glucose in the culture medium and is the result of genetic derepression of E. coli genes involved in proprionate metabolism.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Characterization of a gene cluster responsible for the biosynthesis of anticancer agent FK228 in Chromobacterium violaceum no. 968
Article Abstract:
The study presents an identification and characterization of a gene cluster responsible for the biosynthesis of anticancer agent FK228 in Chromobacterium violaceum no. 968. The acclimation of such an efficient genetic system creates a pathway for the generation of structural analogs as anticancer drug candidates.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Cloning, molecular analysis, and expression of the polyhydroxyalkanoic acid synthase (phaC) gene from Chromobacterium violaceum
Article Abstract:
The polyhydroxyalkanoic acid (PHA) synthase of Chromobacterium violaceum has significant sequence homology with other PHA synthases. The enzyme is the product of the phaC(sub Cv) gene. PHAs are polymers produced by some bacteria that could be used as biodegradable plastics.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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- Abstracts: Transcriptional regulation of the Escherichia coli rhaT gene. The gene yghK linked to the glc operon of Escherichia coli encodes a permease for glycolate that is structurally and functionally similar to L-lactate permease
- Abstracts: Cell surface properties of organic solvent-tolerant mutants of Escherichia coli K-12. Appearance of a stress-response protein, phage-shock protein A, in Escherichia coli exposed to hydrophobic organic solvents
- Abstracts: Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of poly(3-hydroxybutyrate) in Escherichia coli
- Abstracts: Identification of novel mutations in the ryanodine-receptor gene (RYR1) in malignant hyperthermia: genotype-phenotype correlation
- Abstracts: Identification of novel mutations in the ryanodine-receptor gene (RYR1) in malignant hyperthermia: genotype-phenotype correlation. part 2