Proline iminopeptidase gene from Xanthomonas campestris pv. citri
Article Abstract:
Proline iminopeptidase is produced by the cleavage of the N-terminal proline in certain proteins. In the Gram-negative bacteria Xanthomonas campestris pv. citri, the enzyme is coded by the pip gene. Nucleotide sequencing revealed a 939 base pair open reading frame encoding a protein with a molecular mass of 35126 daltons. However, SDS-PAGE identified a molecular mass of 35 kilodaltons and 120 kilodaltons by gel filtration, indicating that the enzyme is a multimer. Amino acid sequence analysis showed a 47% homology to the Pip protein of Neisseria gonorrhoeae.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Binding-protein-dependent arginine transport in Pasteurella haemolytica
Article Abstract:
Arginine transport in Pasteurella haemolytica occurs through a periplasmic binding-protein-dependent transport system. L-arginine is primary ligand for the leucotoxin-associated permease LapT. When present in 1000-fold, excess D-arginine and citrulline compete with arginine for binding to LapT, indicating that the ligand side chain length is critical. However, homoarginine and histidine are poor competitors. Thus, LapT permease specifically transports L-arginine.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Comparison of the azoreductase and nitroreductase from Clostridium perfringens. Regulation and characterization of two nitroreductase genes, nprA and nprB, of Rhodobacter capsulatus
- Abstracts: Nanoscale investigation of pathogenic microbial adhesion to a biomaterial. Metamorphosis of scleratinian coral in response to microbial biofilms
- Abstracts: Coupling of functional gene diversity and geochemical data from environmental samples. Comparisons of protocols for decontamination of environmental ice samples for biological and molecular examinations
- Abstracts: Variation in the human genome. Evolutionary developmental biology: where is it going? Novartis Foundation symposium: genetics and tuberculosis, Cape Town, November 18-20, 1997
- Abstracts: Role of LPS length in clearance rate of bacteria from the bloodstream in mice. A chromosomal ars operon homologue of Pseudomonas aeruginosa confers increased resistance to arsenic and antimony in Escherichia coli