Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif

Article Abstract:

Four promoters from the corynebacterial phi-GA1 phage and 18 promoters from the Sau3A sequences of Corynebacterium glutamicum have been identified by cloning the sequences in the pEKpICm promoter probe vector. The transcriptional start (TS) regions of the promoters have also been identified. The promoters contain conserved sequences in the 35 bp and 10 bp area upstream of the TS region. These sequences are similar to the consensus sequences in Gram-positive and Gram-negative bacteria. The promoters are capable of stimulating the transcription of cat in Escherichia coli.

Observations, Identification and classification, Promoters (Genetics), Amino acid sequence, Amino acid sequencing

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Correlation of isocitrate lyase activity and riboflavin formation in the riboflavin overproducer Ashbya gossypii

Article Abstract:

Isocitrate lyase (ICL) activity and riboflavin formation were studied in an attempt to detect a possible correlation. An ICL mutant of Ashbya gossypii was isolated and characterized to show such correlation, which was observed on soybean oil. The use of glucose as a source of carbon showed that riboflavin overproduction commenced in the late growth phase upon the exhaustion of glucose. ICL activity was parallel, reaching a maximum of 0.41 U (mg protein) super -1. The synthesis of vitamin B2 from the intracellular reserve fat is suggested.

Microbial mutation, Fungi, Pathogenic, Pathogenic fungi

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The ribR gene encodes a monofunctional riboflavin kinase which is involved in regulation of the Bacillus subtilis riboflavin operon

Article Abstract:

A study was conducted to analyze the cloning and sequencing of a 3.5 kb EcoRI-BamHI fragment of Bacillus subtilis chromosomal DNA supporting the ribR gene in the B. subtilis-Escherichia coli shuttle vector pCB20. The ribR gene was amplified by polymerase chain reaction before being cloned and expressed in Escherichia coli. Results indicated that that the ribR gene is part of an operon. Findings also showed a mutation that is regulatory and promotes increases in the production of the ribR gene product in cells.

DNA, Cells (Biology), Escherichia coli, Bacillus subtilis, Cells

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