Promoters of the broad host range plasmid RK2: analysis of transcription (initiation) in five species of Gram-negative bacteria

Article Abstract:

Twelve promoter fragments from plasmid RK2, a broad host range plasmid of the IncP group, were fused to reporter genes and expressed in five species of Gram-negative bacteria to investigate the basis of RK2's broad host range expression system. No consistent trends were detected for any of the promoter-host combinations. Furthermore, the transcriptional start sites for some promoters differed in different hosts. These results indicate that there is no single mechanism to account for the ability of RK2 to express its genes in diverse host species.

Analysis, Gram-negative bacteria, Plasmids, Genetic transcription, Transcription (Genetics)

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Replication of bacteriophage lambda in the Escherichia coli dnaA Delta-rac hosts

Article Abstract:

Research was conducted to examine the role of the Escherichia coli dnaA gene function in the replication of bacteriophage lambda. Results were supported by subsequent researches conducted on the subject. DnaA protein was revealed to stimulate transcription from the phage p(sub R) promoter which plays a crucial role in regulating lambda DNA replication. The DnaA protein was also shown to bind to many regions along the whole lambda genome.

Escherichia coli, Chromosome replication, Bacteriophage lambda

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Involvement of the host initiator function dnaA in the replication of coliphage gamma

Article Abstract:

The initiation of coliphage lambda DNA replication is found to be dependent on the host initiator function dnaA, when the lambdoid prophage Rac is not present. The replication is initiated when the existence of Rac is compensated by the absence of dnaA function. The Rac is associated with the mechanisms of the switch from theta to sigma mode of the lambda phage DNA replication.

DNA, Bacteriophages

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