Properties and gene structure of a bifunctional cellulolytic enzyme (CelA) from the extreme thermophile 'Anaerocellum thermophilum' with separate glycosyl hydrolase family 9 and 48 catalytic domains
Article Abstract:
A large cellulolytic enzyme (CelA) which can hydrolyze microcrystalline cellulose was extracted from the extremely thermophilic, cellulolytic bacterium 'Anaerocellum thermophilum.' The enzyme had significant activity towards Avicel and was most active towards soluble substrates. The enzyme was found to be most closely related to CelA of Caldicellulosiruptor saccharolyticus. Because of the substantially improved Avicelase activity of full-length CelA, exoglucanase activity may be attributed to the C-terminal family 48 catalytic domain.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1998
User Contributions:
Comment about this article or add new information about this topic:
Polythionate degradation by tetrathionate hydrolase of Thiobacillus ferrooxidans
Article Abstract:
The hydrolysis of polythionate by cell-free extracts of Thiobacillus ferrooxidans and the purification of tetrathionate hydrolase were presented. Tetrathionate hydrolase is the enzyme responsible for hydrolysis which was purified to homogeneity using the spectrophotometric assay. Thiosulfate, sulfur and sulfate were the end products of polythionate degradation. The enzyme activity was measured during the purification procedure which is based on the increase of absorbance in the UV region.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Crystal structure of human cytosolic phospholipase A2 reveals a novel topology and catalytic mechanism. Secretory phospholipase A2 generates the novel lipid mediator lysophosphatidic acid in membrane microvesicles shed from activated cells
- Abstracts: Production of a DPP activity gradient in the early drosophila embryo through the opposing actions of the SOG and TLD proteins
- Abstracts: Pilus biogenesis gene, pilC, of Neisseria gonorrhoeae: pilC1 and pilC2 are each part of a larger duplication of the gonococcal genome and share upstream and downstream homologous sequences with opa and pil loci
- Abstracts: Effects of cadmium and of YAP1 and CAD1/YAP2 genes on iron metabolism in the yeast Saccharomyces cerevisiae. Analysis of lysine-dependent yeast sporulation: a decrease in cyclic AMP is not required for initiation of meiosis and sporulation in Saccharomyces cerevisiae
- Abstracts: Peptides 14VIDLL18 and 96FEAAAL101 defined as epitopes of antibodies raised against amino acid sequences of enterotoxigenic Escherichia coli colonization factor antigen I fused to Salmonella flagellin