Protein-mediated adhesion of Lactobacillus acidophilus BG2FO4 on human enterocyte and mucus-secreting cell lines in culture
Article Abstract:
The adhesion of humanLactobacillus acidophilus BG2FO4 to enterocytelike Caco-2 and HT-29 cell lines and to the mucus of mucus-secreting HT29-MTX cells were studied. Electron microscopy showed that BG2FO4 interacted with the apical microvilli of Caco-2 cells without cell damage, as well as with mucus of mucus-secreting cells. Binding was found to require an adhesion-promoting factor which was detected inthe supernatant of L. acidophilus cultures. Partial characterization of the factor showed that it is proteinaceous, and may interact with the carbohydrateson the bacterial cell wall, forming a bridge between bacteria and eukaryotic cell receptors.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Characterization of the tre locus and analysis of trehalose cryoprotection in Lactobacillus acidophilus NCFM
Article Abstract:
The tre locus in Lactobacillus acidophilus was characterized to determine whether or not intracellular uptake of trehalose contributes to cryoprotection and cells subjected to repeated freezing and thawing cycles were monitored for survival in the presence of various concentrations of trehalose. Trehalose uptake was found to be significantly reduced in the transporter mutant but unaffected in the hydrolase mutant and the cryoprotective effect of trehalose was reduced in these mutants suggesting that intracellular transport and hydrolysis contribute significantly to cryoprotection.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Functional analysis of putative adhesion factors in Lactobacillus acidophilus NCFM
Article Abstract:
The complete genome sequence of Lactobacillus acidophilus NCFM is analyzed in order to identify genes involved in adhesion and open reading frames (ORFs) similar to genes implicated previously in adhesion are targeted for insertional inactivation. This study has shown that multiple cell surface proteins in L. acidophilus NCFM can individually contribute to the organism's ability to attach to intestinal cells in vitro.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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