Utilization of cyanide as a nitrogenous substrate by Pseudomonas fluorescensNCIMB 11764: evidence for multiple pathways of metabolic conversion
Article Abstract:
The metabolism of cyanide by Pseudomonas fluorescens NCIMB 11764 was studied. Growth of P. fluorescence with cyanide as the sole nitrogen source was accomplished by using a batch-fed procedure, wherein cyanide was added at carefully timed intervals. Analysis of the metabolites showed that cyanide is is converted to carbon dioxide and ammonia. In addition, higher concentrations of cyanide were converted to formamide and formate, which were also formed during prolonged anaerobic incubation. The results indicate that P. fluorescence NCIMB 11764 can utilize cyanide via two metabolic pathways, one in presence of oxygen, and the other, under anaerobic conditions.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Accumulation of alpha-keto acids as essential components in cyanide assimilation by Pseudomonas fluorescens NCIMB 11764
Article Abstract:
Research was conducted to examine the accumulation of alpha-ketoglutarate acids as important components of cyanide assimilation by Pseudomonas fluorescens NCIMB 11764. Results demonstrate that cyanide loss from cultures independent of the one catalyzed by CNO takes place through a chemical reaction with excreted alpha-keto acids. Findings provide a foundation for a new model of cyanide utilization wherein alpha-keto takes on a critical role.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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Enzymatic assimilation of cyanide via pterin-dependent oxygenolytic cleavage to ammonia and formate in Pseudomonas fluorescens NCIMB 11764
Article Abstract:
Research describes purification and characterization of cyanide oxygenase involved in cyanide assimilation by Pseudomonas fluorescens NCIMB 11764. Results demonstrate cyanide is enzymatically converted to formate and ammonia by a pterin-dependent oxygenative mechanism, with ammonia serving as the nitrogen source to the bacterium.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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