Protein-protein interactions: methods for detection and analysis

Article Abstract:

The effects of protein-protein interactions include protein kinetic property variations, substrate channeling, generation of new binding sites, protein inactivation and altering protein-substrate specificity. Several biochemical molecular biological and genetic techniques such as protein affinity chromatography, coimmunoprecipitation, phage display, protein probing and the isolation of synthetic mutants help study these interactions. The binding constants that determine the strength of the interactions can also be determined by biochemical methods. Leucine zipper, SH3 and SH2 domains are some of the well-characterized domains that are essential for protein-protein interactions.

Analysis, Binding sites (Biochemistry), Active sites (Biochemistry)

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Fungal lipopeptide mating pheromones: a model system for the study of protein prenylation

Article Abstract:

The lipopeptide pheromones that mediate mating between fungi may provide models for protein prenylation studies. The attachment of isoprenoid groups to peptides, a comon event in lipopeptide synthesis, is a modification that occurs during processing of proteins such as nuclear lamins and Ras. The a-factor, a lipopeptide pheromone in Saccharamyces cerevisiae, is secreted in a process that involves the yeast version of the mammalian multidrug resistance P- glycoprotein.

Pheromones

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Protein trafficking in kinetoplastid protozoa

Article Abstract:

Protein trafficking in kinetoplastid protazoa varies from that in higher eukaryotes. Kinetoplastid signal sequences for subcellular compartments are more degenerate. Other components of trafficking, such as clathrin-coated vesicles and recycling receptors, are absent. Kinetoplastids also have some unique trafficking properties that may provide targets for drug treatment of infections caused by these organisms.

Eukaryotic cells, Cells (Biology), Eukaryotes, Biological transport, Kinetoplastida

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