Proteinase overproduction in Lactococcus lactis strains: regulation and effect on growth and acidification in milk
Article Abstract:
The genetic regulation of SK11 proteinase production and control of lactococci growth rate in milk were described. Multicopy plasmids containing complete and 3'-deleted sections of the proteinase gene of Lactococcus lactic subspecies cremoris SK11 were constructed and introduced into lactose fermenting Prt- lactoccal strains. Results showed that proteinase production were regulated by the medium composition for all strains. Consequently, lactoccal growth and acidification rate were enhanced by the overproduction and of secreted or envelope-bound SK11 proteinase.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Genetic marking of Lactococcus lactis shows its survival in the human gastrointestinal tract
Article Abstract:
The Lactococcus lactis cells consumed in dairy products, are able to survive high conjugated bile salts found in gastrointestinal tract provided they pass within 3 days of consumption indicating that Lactococcus strains can be used in the formulation of oral vaccines. The stability of Lactococcus lactis in human gastrointestinal tract was studied using genetically marked Lactococcus lactis strains and monitoring the survival and persistence of chromosomal DNA by conventional plating method.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Detection and localization of synthropic propionate-oxidizing bacteria in granular sludge by in situ hybridization using 16S rRNA-based oligonucleotide probes
Article Abstract:
Fluorescently labeled probes are used together with 16S rRNA-based probes for Bacteria and Archaea to detect and localize microorganisms in the granules of two lab-scale upflow anaerobic sludge blanket reactors fed with either sucrose or a mixture of volatile fatty acids. Results suggest that the hybridization of granular sludge with fluorescent probes is a powerful tool for studying structure-function relationships in situ.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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