Purification and biochemical characterization of mutacin I from the group I strain of Streptococcus mutans, CH43, and genetic analysis of mutacin I biosynthesis genes
Article Abstract:
A description of mutacin I biosynthesis is presented. The biosynthesis gene locus contains 14 genes and the resultant protein is 24 amino acids long with a molecular mass of 2,364 Da.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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Functional analyses of the promoters in the lantibiotic mutacin II biosynthetic locus in Streptococcus mutans
Article Abstract:
DNA sequence analyses of the promoters in the lantibiotic mutacin II biosynthetic locus in Streptococcus mutans were conducted. The objectives were to gain further insight into the regulatory mechanisms for mutacin II production and to determine the transcription units in the mutacin locus. Results indicated promoter-like structures upstream of mutA and mutR and a rho-independent transcription terminator-like seqence between mutA and mutR.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Purification of mutacin III from group III Streptococcus mutans UA787 and genetic analyses of mutaxi III biosynthesis genes
Article Abstract:
The cloning and sequencing of the mutacin III biosynthesis genes were conducted using information from the conserved sequence derived from various other lantibiotics. The isolation and purification of mutacin III from a modified semisolid culture medium were also investigated. The molecular mass of the purified peptide was calculated using laser desorption mass spectrophotometry and observed to be 2,266.43 Da, which is in agreement with the hypothesis that mutacin III has posttranslational modifications that are similar to those of the lantibiotic epidermin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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