Purification and characterization of L-methionine gamma-lyase from Brevibacterium linens BL2

Article Abstract:

The purification of L-Methionine-gamma-lyase (MGL) from Brevibacterium linens BL2, a bacterium used in the improvement of Cheddar cheese flavor, was conducted. Methanethiol, alpha-ketobutyrate and ammonia were generated by the enzymatic catalysis of the alpha, gamma elimination of methionine. Results suggest that the pure enzyme possessed catalytic properties similar to those of MGL from Pseudomonas putida. The enzyme was found to be active under salt and pH conditions but susceptible to intracellular protease degradation.

Composition, Methionine, Cheddar cheese

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Nucleotide sequence and taxonomical distribution of the bacteriocin gene lin cloned from Brevibacterium linens M18

Article Abstract:

The structural gene from linocin M18, an antilisterial bacteriocin produced by the bacterium Brevibacterium linens M18, is widely distributed in corynebacteria from dairy products. The gene occurs in three Brevibacterium species, five Arthrobacter species and five Corynebacterium species. N-terminal amino acid sequence probes were used to determine the position of the single copy gene, lin, on the chromosomal DNA. The amino acid sequence, composition, and nucleotide sequence are discussed.

Analysis, Nucleotide sequence, Base sequence, Amino acid sequence, Amino acid sequencing

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Growth reduction of Listeria spp. caused by undefined industrial red smear cheese cultures and bacteriocin-producing Brevibacterium linens as evaluated in situ on soft cheese

Article Abstract:

A research was conducted to investigate the dependency of listerial growth on the microbial composition of the undefined surface floras through the inoculation of the cheese surface with wash-off cultures from commercial cheese. A cheese sample was diluted in parallel to determine variations in cell counts resulting from dilution and plating. Results suggested that bacteriocin production of ripening cultures may have contributed to the inhibition of Listeria.

Cell culture, Tissue culture, Listeria, Cottage cheese

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