Purification and characterization of a keratinase from a feather-degrading Bacillus licheniformis strain
Article Abstract:
Bacillus licheniformis PWD-1 is a feather-degrading bacterium capable of using feathers as its primary source of carbon, sulfur and energy. An investigation was conducted to isolate and purify the active keratinase responsible for this process. The results showed that the keratinase is monomeric with a molecular mass of 33 kDa. The keratinase could hydrolyze a broader range of substrates than most proteases, and had optimum activities at pH 7.5 and at 50 degress celsius. The purified enzyme will be useful for improving the quality of feather meal.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Survival of Bacillus licheniformis in seawater model ecosystems
Article Abstract:
The survival of Bacillus licheniformis DSM 113 introduced into the laboratory microcosms and mesocosms was evaluated. The model organism was studied by immunoflourescence spectroscopy and nonselective plating in combination with colony blotting. Results showed that microcosm models were effective in determining single-factor influence. On the other hand, mesocosms allowed the evaluation of the effect of foreign microrganism introduction on the structure and function of ecosystem.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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An altered Pseudomonas diversity is recovered from soil by using nutrient-poor Pseudomonas-selective soil extract media
Article Abstract:
The NAA 1:100 culture medium is more effective than Gould's S1 medium when isolating Pseudomonas from low-nutrient soil. The NAA 1:100 medium resulted in higher CFU counts when tested on four Danish soil samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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