Purification and characterization of a new bacteriocin isolated from Carnobacterium sp

Article Abstract:

A new bacteriocin was isolated and purified from Carnobacterium sp. isolated from fish. The bacteriocin was designated as carnocin UI49 and contained 35 to 37 amino acid residues, 55% to 60% of which are nonpolar. Strong evidence indicated the presence of lanthionine. Sequence analysis revealed seven N-terminal amino acids which were non-homologous to any other sequenced bacteriocins from lactic acid bacteria. Mass spectrometry revealed a molecular weight of 4,635 Da. The mode of action was found to be bactericidal, with optimum activity between pH 2.0 and 8.0. The results indicate that carnocin UI49 is a lantibiotic.

Analysis, Bacteriocins

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Identification and characterization of Leuconostoc carnosum, associated with production and spoilage of vacuum-packaged, sliced, cooked ham

Article Abstract:

Research was conducted to identify type A lactic acid bacteria (A LAB) to the species level and to characterize the 100 isolates possessing the type A EcoRI and HindIII ribopatterns. Ribotyping was employed in the creation of a reference strain library that will be used with phenotypic data for the species identification of LAB. Results indicate that ribotyping is essential in the identification of Leuconostoc carnosum. However, its discriminatory power is not sufficient for strain characterization.

Environmental aspects, Food, Bacteria, Food microbiology, Food spoilage

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A food-grade process for isolation and partial purification of bacteriocins of lactic acid bacteria that uses diatomite calcium silicate

Article Abstract:

A porous calcium silicate produced by the hydrothermal reaction of diatomite, hydrated lime and water is used in extracting bacteriocins of the lactic acid bacteria from growth media. Analyses of the extracted bacteriocins indicate that the porous medium, called Micro-Gel, allows higher levels of desorption increasing surfactant concentration.

Sanitary microbiology

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