Purification and characterization of alkaline xylanases from Bacillus polymyxa
Article Abstract:
The purification and characterization of three xylanases from Bacillus polymyxa were reported. Xylanases X34C and X34E are closely related alkaline enzymes of 34 kDa and a pI of greater than 9.3, and xylanase X22 is a highly active enzyme of 22 kDa and a pI of 9.0. The enzymes were nondebranching endo-beta-xylanases. Xylose is a major product of xylan hydrolysis by xylanases X34C and X34E, but this is not an end product by xylanase X22. However, these enzymes do not hydrolyze xylobiose.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Purification and characterization of a new agarase from a marine bacterium, Vibrio sp. strain JT0107
Article Abstract:
The purification and characterization of a new agarase from Vibrio sp. strain JT0107 were reported. Agarase 0107 is estimated to have a molecular mass of 107 kDa. It is an endo-type beta-agarase that hydrolyzes agarose and neoagarohexaose, but could not hydrolyze kappa-, lambda- and iota-carrageenans. The optimum temperature and pH are 30 degrees C and pH 8, respectively. This enzyme may be of practical application in gene technology.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Relative contributions of bacteria, protozoa, and fungi to in vitro degradation of orchard grass cell walls and their interactions
Article Abstract:
Results show that plant cell wall degradation in the rumen is by far due to the fungal activity followed by that of anaerobic bacteria with no effect from the protozoal activity. However, in mixed cultures, both antagonistic and synergistic interactions are observed leading to overall decrease in cellulolysis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Purification and characterization of two alpha-L-arabinofuranosidases from Streptomyces diastaticus. Exo-arabinanase of Penicillium chrysogenum able to release arabinobiose from alpha-1,5-L-arabinan
- Abstracts: Purification and partial characterization of a tripeptidase from Pediococcus pentosaceus K9.2. Development and characterization of lactose-positive Pediococcus species for milk fermentation
- Abstracts: Purification and characterization of a fibrinolytic enzyme produced from Bacillus sp. strain CK 11-4 screened from Chungkook-Jang
- Abstracts: Molecular characterization of cycloinulooligosaccharide fructanotransferase from Bacillus macerans. Genetic and biochemical characterization of a highly thermostable alpha-L-arabinofuranosidase from Thermobacillus xylanilyticus
- Abstracts: Phylogenetic diversity of rhizobial strains nodulating Robinia pseudoacacia L