Purification and characterization of a novel 5-oxoprolinase (without ATP-hydrolyzing activity) from Alcaligenes faecalis N-38A
Article Abstract:
Research was conducted to examine the purification and characterization of a novel 5-oxoprolinase (without ATP-hydrolyzing activity) from Alcaligenes faecalis N-38A. The novelty of the 5-oxoprolinase was analyzed based on the decyclization reaction of L-pyroglutamate and the cyclization reaction of L-glutamate. Results provide strong evidence that the 5-oxoprolinase found in a cell extract of strain N-38A is a novel type of 5-oxoprolinase.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
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Novel bifunctional hyperthermostable carboxypeptidase/aminoacylase from Pyrococcus horikoshii OT3
Article Abstract:
An enzyme has been isolated from the thermophilic archaeon Pyrococcus horikoshii OT3 which is a new zinc-dependent metalloenzyme that has a structure similar to carboxypeptidase but a mechanism similar to aminoacylase. This indicates that carboxypeptidase and aminoacylase may have a common evolutionary origin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Molecular cloning, sequencing, and expression in Escherichia coli of the gene encoding a novel 5-oxoprolinase without ATP-hydrolyzing activity from Alcaligenes faecalis N-38A
Article Abstract:
A 5-oxoprolinase from Alcaligenes faecalis N38A with no sequence similarity to any other known protein is described. It is a zinc metalloenzyme with 402 amino acid residues and a mass of 46,163 Da.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2000
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