Purification and characterization of alpha-L-arabinofuranosidase from Bacillus stearothermophilus T-6
Article Abstract:
Anion-exchange and hydrophobic interaction chromatographies help purify alpha-L-arabinofuranosidase produced by Bacillus stearothermophilus T-6. Analysis of the enzyme reveals the molecular weight as 256,000 and gel electrophoresis and gel filtration reveal the presence of four identical subunits in the enzyme. Cofactors are not necessary for the activity of the enzyme and the presence of Hg2+ inhibits the activity. The N-terminal sequence of the enzyme is compared with that of alpha-L-arabinofuranosidase from Streptomyces lividans 66.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Purification and characterization of a thermostable xylanase from Bacillus stearothermophilus T-6
Article Abstract:
An extracellular xylanase produced by Bacillus stearothermophilus T-6 optimally bleaches pulp at pH9 and at 65 degrees Celsius. The purification and characterization of the enzyme xylanase T-6 is reported. Xylan is degraded to xylose and xylobiose by Xylanase T-6 and endoxylanase. The protein is protected by xylane from inactivation by N-bromosuccinimide. The N-terminal sequence of the first 45 amino acids of the enxyme revealed high homology with the N-terminal region of xylanase A from the alkalophililc Bacillus sp strailn C-125.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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A two-component system regulates the expression of an ABC transporter for xylo-oligosacchaillus stearothermophilus.rides in Geobac
Article Abstract:
A study was conducted to show that in Geobacillus stearothermophilus strain T-6, a two-component sensing system, encoded by xynDC, positively regulates the expression of an adjacent ABC transporter system (xynEFG) designed for the update of xylo-oligosaccharides. The result data show that XynEFG constitutes an ABC transport system for xylo-oligosaccharides and its transcription is negatively regulated by XylR and activated by the response regulator XynC which is part of a two-component sensing system.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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