Purification and characterization of an extracellular poly(L-lactic acid) depolymerase from a soil isolate, Amycolatopsis sp. strain K104-1
Article Abstract:
Findings are presented on the ideal conditions for the biodegradation of poly(L-lactic acid), or PLA, a material being developed as a biodegradable and renewable plastic. A purified enzyme derived from the K104-1 and K104-2 strains of the Amycolatopsis bacterium degraded both solid and emulsion films of high-molecular-weight PLA into lactic acid, with the optimum temperature between 55 to 60 degrees Celsius and the optimum pH at 9.5.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Cloning, expression and cell surface localization of Paenibacillus sp. strain W- 61 xylanase 5, a multidomain xylanase
Article Abstract:
The classification of the bacterium, Aeromonas caviae W-61 to Paenibacillus sp. strain W-61 is cloned and expressed on the basis of the nucleotide sequence of the 16S rRNA gene emended and the xyn5 gene encoding xylanese 5 (Xyn5) in Escherichia coli. Findings concluded that Paenibacillus sp. strain W-61 Xyn5 is a cell surface anchored modular xylanase possessing a functional cellulose binding module and SLH domains.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Amylomaltase of Pyrobaculum acrophilum IM2 produces thermoreversible starch gels
Article Abstract:
The amylomaltase-encoding gene from the hyperthermophilic archaeon Pyrobaculum acrophilum IM2 was cloned and expressed in Escherichia coli, and the gene product (PyAMase) was characterized. Acting on gelatinized food-grade potato starch, PyAMase produced a thermoreversible starch product with gelatin-like properties.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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