Purification and characterization of phenylacetaldehyde reductase from a styrene-assimilating Cornyebacterium strain, ST-10
Article Abstract:
The Corynebacterium strain St-10 capable of gaseous styrene assimilation was used to purify a novel phenylacetaldehyde reductase 50-fold to homogeneity. The enzyme, which was observed to be vital in in vivo styrene metabolism, featured four identical subunits and had a 155,000 molecular weight. The decrease of several ketones, aldehydes and phenylacetaldehyde was achieved through the enzyme. However, the enzyme failed to promote the dehydrogenation of 2-phenylethanol.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Engineering of Phenylacetaldehyde reductase for efficient substrate conversion in concentrated 2-Propanol
Article Abstract:
Phenylacetaldehyde reductase (PAR) is suitable for the conversion of various aryl ketones and 2-alkanones to corresponding chiral alcohols. 2-Propanal acts as a substrate solvent and hydrogen donor of coupled cofactor regeneration during the conversion of substrate catalyzed by PAR.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Purification and cDNA cloning of NADPH-dependent aldoketoreductase, involved in asymmetric reduction of methyl 4-bromo-3-oxobutyrate, from penicillium citrinum IFO4631
Article Abstract:
Penicillium citrinum was found to catalyze the reduction of methyl 4-bromo-3-oxobutyrate to methyl (S)-4-bromo-3-hydroxybutyrate with high optical purity. From the strain, a cDNA clone encoding a novel NADPH-dependent alkyl 4-halo-3-oxobutyrate reductase (KER) was isolated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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