Quantification of enterococci and human adenoviruses in environmental samples by real-time PCR
Article Abstract:
The development of real-time PCR methods for quantification of both human adenoviruses and enterococci in environmental samples is described. The real time PCR methods showed a greater sensitivity in detection of adenoviruses in sewage samples than the viral plaque assay and in detection of entercocci in coastal waters than the bacterial culture method.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Seasonal detection of human viruses and coliphage in Newport bay, California
Article Abstract:
The occurrence and the distribution of fecal indicator bacteria (FIB), F coliphage, and human adenovirus and enterovirus is investigated for an entire year at 15 locations around the Newport Bay watershed. The detection of human viruses depends on a distribution pattern that is the opposite of that of FIB and coliphage.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2007
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Prevalence of cholera toxin genes (ctxA and zot) among non-O1/O139 Vibrio cholerae strains from Newport Bay, California
Article Abstract:
The diversity and prevalence of virulence genes ctxA and zot among non- O1/O139 V. cholerae isolates from the Newport Bay, California, watershed region of nonepidemicty is reported. The findings suggest that ctxA is prevalent in the region of nonepidemicty and is likely to have an environmental origin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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- Abstracts: Real-time PCR for quantification of Giardia and Cryptosporidium in environmental water samples and sewage. Infectious Cryptosporidium parvum oocysts in final reclaimed effluent
- Abstracts: Detection and quantification of Wallemia sebi in Aerosols by real-time PCR, conventional PCR, and cultivation
- Abstracts: Quantitative real-time PCR assays for detection of human adenoviruses and identification of Serotypes 40 and 41
- Abstracts: Application of a specific and sensitive radiometric assay for microbial lipase activities in marine water samples from the lagoon of Noumea
- Abstracts: Genetic diversity of Campylobacter jejuni isolates from farm animals and the farm environment. Cyanophage diversity, inferred from g20 gene analyses, in the largest natural lake in France, Lake Bourget