Quantitative real-time PCR assays for detection of human adenoviruses and identification of Serotypes 40 and 41
Article Abstract:
A quantitative real-time TaqMan PCR assay is developed for detection of human adenoviruses (HAdV), followed by a second real-time PCR assay, the fluorescence resonance energy transfer (FRET) that targets the adenovirus fiber gene. Application of these assays for detection of adenoviruses and type-specific identification of AdV40 and AdV41 is expected to be useful for identifying viruses in environmental and clinical samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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Detection of infectious adenovirus in cell culture by mRNA reverse transcription-PCR
Article Abstract:
The reverse transcription (RT)-PCR detection of mRNA are developed and evaluated in cell culture to assay infectious adenoviruses (Ads) by using Ad type 2 (Ad2) and Ad41 as models. The studies suggest that mRNA detection by RT-PCR assay in inoculated cell cultures is a very sensitive, specific and rapid method that detects infectious Ads in water and other environmental samples.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
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Use of cell culture-PCR assay based on combination of A549 and BGMK cell lines and molecular identification as a tool to monitor infectious adenoviruses and enteroviruses in river water
Article Abstract:
A cell culture assay performed with A549 and Buffalo green monkey kidney (BGMK) cells, followed by cell culture RT-multiplex nested PCR and molecular identification is described. The usefulness of this method for monitoring infectious adenoviruses and enteroviruses in river water is evaluated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2004
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