Quantitative assessment of in planta distribution of metabolic activity and gene expression of an endophytic fungus
Article Abstract:
Extraction of endophyte-associated glucoronidase (GUS) activity from plant tissue was done using a newly developed method employing the extraction of ground lyophilized material by four cycles of sonication. The in planta GUS expression by the fungi was monitored by fluorescence microscopy and it was found that the GUS gene is constitutively expressed in the fungal hyphae. The quantitation of the GUS activity revealed that the concentration of endophyte in every plant part is so regulated that a threshold of the activity is not surpassed and consistent distribution pattern is maintained.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
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Endophytic fungi in indigenous Australasian grasses associated with toxicity to livestock
Article Abstract:
A study was conducted to describe a Neotyphodium endophyte found in New Zealand specimens of Echinopogon ovatus, the isolation of the endophyte from infected seeds, identification of the endophyte as a Neotyphodium specie on the basis of its cultural and serological characteristics and the presence of indole-diterpenoid and loline alkaloids in E. ovatus. Grass endophytes were thought to be transmitted through the seed. Endophyte-free plants did not become infected with Neotyphodium endophytes. Results suggested that endophyte infection confers advantages on E. ovatus.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1998
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In planta regulation of extension of an endophytic fungus and maintenance of high metabolic rates in its mycelium in the absence of apical extension
Article Abstract:
There appears to be some type of regulatory system inside the leaves of the ryegrass Lolium perenne that controls the growth of its fungal endophytic symbiont, Neotyphodium lolii. Hyphal extension ceases when the leaf stops growing even though the hyphae remain metabolically active.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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