Rapid detection of members of the family Enterobacteiaceae by a monoclonal antibody
Article Abstract:
Monoclonal antibodies were produced against Escherichia coli O14:K7 to detect the enterobacterial common antigen. Six clones were produced, all of which had similar reactivities based on immunoblotting. One of the clones, CX9/15, was tested on a variety of microbial species. CX9/15 was able to recognize all species of the Enterobacteriaceae tested except for Erwinia chrysanthemi. Of 125 gram-negative bacteria not belonging to the Enterobacteriaceae, 117 were not detected. Cross reaction was also observed with Plesiomonas shigelloides, Aeromonas hydrophila and Aeromonas sobria.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1992
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Rainfall-induced release of fecal coliforms and other manure constituents: Comparison and modeling
Article Abstract:
The release of fecal coliforms (FC), chloride ([Cl.sup.-]), organic carbon (OC), and water-soluble phosphorus (P) from bovine manure applied on slopes with different soil textures and surface covers under simulated rainfall are compared and the applicability of three models to describe the kinetics of [Cl.sup.-], FC, OC and P release are assessed. The kinetics of the FC release from manure is similar to the release kinetics of P and OC and the Bradford-Schijven model is recommended to simulate the release of manure constituents.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
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Comparison of the chemical assay, bioassay, enzyme-linked immunoabsorbent assay, and dot blot hybridization for detection od aerobactin in members of the family Enterobacteriaceae
Article Abstract:
The results of three phenotypic assays were compared to determine the best method for detecting aerobactin in members of the family Enterobacteriaceae. The sensitivity and specificity of a chemical assay, a cross-feeding bioassay and an enzyme-linked immunosorbent assay (ELISA). The specific probe for the aerobactin genes was the dot blot hybridization assay. ELISA was found to be the best detector for aerobactin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
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