Specific detection and confirmation of Campylobacter jejuni by DNA hybridization and PCR
Article Abstract:
A specific DNA probe, pDT1720, is used to detect and confirm the presence of Campylobacter jejuni in spiked food samples and oligonucleotide segments from this probe are used as primers in polymerase chain reaction (PCR) studies conducted on milk and chicken rinse samples. Both methods are rapid, specific and sensitive detectors of C. jejuni contamination of food. Further, the PCR method has the potential to quantify bacterial counts in contaminated milk and chicken.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
Microbial ecology of Campylobacter jejuni in a United Kingdom chicken supply chain: intermittent common source, vertical transmission, and amplification by flock propagation
Article Abstract:
Vertical transmission of Campylobacter jejuni in broiler chicken-rearing farms in England takes place between and within flocks and the isolation rate of the bacterium is dependent on the hatchery supplying chicks. A common source of the Campylobacter is evident in the absence of diversity of types and the intermittent high positivity of sheds. The frequency distribution of Campylobacter types for chickens supplied by 2 hatcheries exhibit distinct dissimilarity.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Temperature-driven campylobacter seasonality in England and Wales
Article Abstract:
The relationship between seasonal variation in human campylobacter infection in England and Wales and environment conditions is investigated to obtain new insights with respect to disease transmission. Public health interventions should not be restricted to food-borne approaches, and the epidemiology of the seasonal peak in human campylobacter infections may best be understood through studies in young children.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Identification and quantification of Bifidobacterium species isolated from food with genus-specific 16S rRNA-targeted probes by colony hybridization and PCR
- Abstracts: Physical and genetic map of the genome of Campylobacter upsaliensis. Campylobacter upsaliensis exerts a cytolethal distending toxin effect on HeLa cells and T lymphocytes
- Abstracts: Stage-specific regulation of murine Hsp68 gene promoter in preimplantation mouse embryos. Developmental and tissue-specific regulation of the murine cardiac actin gene in vivo depends on distinct skeletal and cardiac muscle-specific enhancer elements in addition to the proximal promoter
- Abstracts: Recombination-dependent mutation in Escherichia coli occurs in stationary phase. Genetic recombination in Escherichia coli: disputation at Cold Spring Harbor, 1946-1996
- Abstracts: Isolation and characterization of isopimaric acid-degrading bacteria from a sequencing batch reactor. Aerobic biodegradation of biphenyl and polychlorinated biphenyls by arctic soil microorganisms