Rapid identification of Vibrio vulnificus on nonselective media with an alkaline phosphatase-labeled oligonucleotide probe
Article Abstract:
A method that allows the immediate division and enumeration of the organism without enrichment or selective media is evaluated. This involved the utilization of an alkaline phosphatase-labeled oligonucleotide probe derived from the DNA sequence within the hemolysin-cytolysin structural gene for detecting Vibrio vulnifucus in environmental samples. The rapid accuracy of the method was demonstrated.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1993
User Contributions:
Comment about this article or add new information about this topic:
Genetic distinctions among clinical and environmental strains of Vibrio vulnificus
Article Abstract:
PCR is used to screen DNA polymorphisms in strains from environmental or clinical sources, and genomic relationships were determined by repetitive extragenic palindromic DNA PCR (rep-PCR) typing. Significant (P<0.01) association was observed for typical 'clinical' or 'environmental' polymorphism profiles based on strain origin.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
Real-time PCR analysis of vibrio vulnificus from oysters
Article Abstract:
A quantitative real- time PCR assay with TaqMan technology is developed. The data indicates that real- time PCR can provide sensitive species- specific detection and enumeration of V. vulnificus in seafood.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2003
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: In situ identification of cyanobacteria with horseradish peroxidase-labeled, rRNA-targeted oligonucleotide probes
- Abstracts: Identification of individual prokaryotic cells by using enzyme-labelled, rRNA-targeted oligonucleotide probes
- Abstracts: Whole-cell hybridization of Frankia strains with fluorescence- or digoxigenin-labeled, 16S rRNA-targeted oligonucleotide probes
- Abstracts: Enhanced utilization of phosphonate and phosphite by Klebsiella aerogenes. Rapid method for analyzing bacterial behavioral responses to chemical stimuli
- Abstracts: Comparison of identification systems for classification of bacteria isolated from water and endothilic habitats within the deep subsurface. part 2