Rapid one-step quantitative reverse transcriptase PCR assay with competitive internal positive control for detection of enteroviruses in environmental samples
Article Abstract:
A quantitative reverse transcriptase PCR (qRT-PCR) assay specifically tailored to detect the viruses in environmental waters is described as human enteroviruses serves as a more accurate indicator of human fecal contamination than conventional bacteriological fecal indicators. The findings reveal that the ability to the assay to identify false negatives and provide improved quantitative assessments of enterovirus concentrations would assist the tracking of human fecal contamination and the assessments of potential public health risk due to enteroviruses in recreational and shellfish harvesting waters.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2006
User Contributions:
Comment about this article or add new information about this topic:
In situ detection of the Clostridium botulinum type C1 toxin gene in wetland sediments with a nested PCR assay
Article Abstract:
Clostridium botulinum type C is an anaerobic, spore-forming bacterium found naturally in the sediments of lakes and marshes. A nested PCR was devised for the detection of C. botulinum type C1 toxin gene in sediments obtained from wetlands where avian botulism outbreaks had or had not occurred. The C1 toxin gene was detected in 16 of 18 sites, indicating both the widespread distribution of C. botulinum type C in wetland sediments and the sensitivity of the detection assay.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1999
User Contributions:
Comment about this article or add new information about this topic:
Sensitive detection of botulinum neurotoxin types C and D with an immunoaffinity chromatographic column test
Article Abstract:
A sensitive and specific immunoassay for the simultaneous detection of Clostridium botulinum type C (BoNT/C) and type and type D neurotoxin is developed. Streptavidin-poly-horseradish peroxidase is used as a conjugate and a precipitating substrate allowed the direct semiquantitative readout of the assay and for a more accurate quantitative detection, the substrate can be eluted and measured in a photometer.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Development and application of 16S rRNA-targeted probes for detection of iron- and manganese-oxidizing sheathed bacteria in environmental samples
- Abstracts: Quantitative real-time PCR assays to identify and quantify fecal Bifidobacterium species in infants receiving a prebiotic infant formula
- Abstracts: Association of transcriptionally silent genes with Ikaros complexes at centromeric heterochromatin. Distinct roles for Drosophila Dicer-1 and Dicer-2 in the siRNA/miRNA silencing pathways
- Abstracts: Captured diversity in a culture collection: Case study of the geographic and habitat distributions of environmental isolates held at the American type culture collection
- Abstracts: Statistical approach for comparison of the growth rates of five strains of Staphylococcus aureus. Rapid biosensor of detection of antibiotic-selective growth of Escherichia coli