Rat gene mapping using PCR-analyzed microsatellites

Article Abstract:

A rat gene map was constructed using 174 distinct rat sequences derived from public data bases to generate sequence tagged microsatellite sites (STMSs) through polymerase chain reaction techniques. Since the 174 loci represented mostly protein coding regions, the resulting map is also a gene map. The analysis showed that STMSs with more than 10 repeats of each motif were more likely to be polymorphic. The microsatellite loci were also highly stable, making them amenable for linkage studies. The results showed that the use of STMSs is a powerful method for resolving the rat gene map.

Genetic aspects, Polymerase chain reaction, Rats, Satellite DNA

---

A general Monte Carlo method for mapping multiple quantitative trait loci

Article Abstract:

The mapping of multiple quantitative trait loci (QTL) may prove difficult especially when dominant markers are used in experiments in outbred populations. To simplify mapping in such situations, a general and flexible Monte Carlo expectation-maximization algorithm was developed. The algorithm is simple to implement in standard statistical software but the computation of data may take considerable time. The algorithm was tested using a three-QTL model of an outbreeding experiment involving dominant markers.

Algorithms, Monte Carlo method, Monte Carlo methods

---

Linkage analysis using co-phenotypes in the BRIGHT study reveals novel potential susceptibility loci for hypertension

Article Abstract:

The development of a score test for the Rice-Holmans model and its application to multiple phenotypic covariates and genome-scan data from the affected sibling pairs in the BRIGHT study are described. This is the first identity-by-descent regression analysis of hypertension and it has shown the value of this approach for the incorporation of additional phenotypic information in genetic studies of complex traits.

Analysis, Phenotype, Phenotypes, Linkage (Genetics), Genetic susceptibility

Similar abstracts:

• Abstracts: Multiple trait analysis of genetic mapping for quantitative trait loci. Maximum likelihood mapping of quantitative trait loci using full-sib families
• Abstracts: A PCR detection method for rapid identification of Paenibacillus larvae. Characterization of bacterial and fungal soil communities by automated ribosomal intergenic spacer analysis fingerprints: biological and methodological variability
• Abstracts: A highly polymorphic degenerate microsatellite for molecular strain typing of Candida krusei. Analysis of the wee gene cluster responsible for the biosynthesis of the polymeric bioemulsifier from the oil-degrading strain Acinetobacter lwoffii RAG-1
• Abstracts: Comparing evolvability and variability of quantitative traits
• Abstracts: Microbial characterization of biological filters used for drinking water treatment. Filtration sizes of human immunodeficiency virus type 1 and surrogate viruses used to test barrier materials

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.