Recombination by replication
Article Abstract:
The initiation of DNA replication in Escherichia coli requires homologous recombination and activity of the PriA replication priming protein. The replication fork created at the D-loops by PriA-catalyzed primosome assembly initiates DNA replication. This is followed by the loading of DnaB helicase onto single-stranded DNA, and RNA primers synthesis for lagging-strand DNA. The invading single-stranded DNA with a3' ends may be used to initiate leading strand synthesis. Both recombination and replication are essential for the repair of exposed double-stranded ends in E. coli.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1996
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Comparison of recombination in vitro and in E. coli cells: measure of the effective concentration of DNA in vivo
Article Abstract:
Chromosomal dimerization and tangling of the chromosomes do not take place in the nucleus though the concentration of DNA in the nucleus is very high which could be because the reactivity of the DNA is much less than the chemical concentration. The large amount of DNA in the nucleus of E. coli cells increases the DNA collision, leading to the formation of nucleoprotein complexes which are necessary for metabolism. However, the behaviour of DNA in vivo may differ from these in vitro results.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1995
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A super new twist on the initiation of meiotic recombination
Article Abstract:
Recombination between homologous chromosomes happens at least 1,000 times more frequently in meiosis than in mitosis. In meiosis, there are 'hot spots' where recombination occurs 10-50 times more frequent than at other sites. SPO11 is related to a novel family of type II toposiomerases and one of the first meiotic-specific genes required for the initiation of meiotic recombination.
Publication Name: Cell
Subject: Biological sciences
ISSN: 0092-8674
Year: 1997
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