Recovery from DNA replicational stress is the essential function of the S-phase checkpoint pathway
Article Abstract:
MEC1 and RAD53 are essential genes for the cell cycle and transcriptional responses to DNA damage and DNA replication blocks. Essential function of these genes has been studied. Mec1 is more important than Rad53 in response to inhibition of DNA synthesis, based on study of viable null alleles. Their lethality defects can be suppressed by greater expression of genes encoding ribonucleotide reductase. However, that is not the case for the checkpoint defects. It appears that the checkpoint pathway plays has an important role in maintaining DNA synthetic capabilities when DNA replication is stressed.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 1998
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Control of ribonucleotide reductase localization through an anchoring mechanism involving WTM1
Article Abstract:
The control of deoxyribonucleotide levels is essential for DNA synthesis and repair that is exerted through regulation of ribonucleotide reductase (RNR) is reported. It suggests that WTM1 acts as a nuclear anchor to maintain nuclear localization of Rnr2/4 complexes outside of S phase and in the presence of DNA damage this association is disrupted and Rnr2/Rnr4 become cytoplasmic, where they join with Rnr1 to form an intact complex.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2006
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Adaptor protein Ste50p links Ste11p MEKK to the HOG pathway through plasma membrane association
Article Abstract:
The regulatory role of Ste50p requires both the N-terminal Sterile alpha motif domain to bind the mitrogen extracellular signal-regulated kinase kinase (MEKK) Ste11p and the C-terminal Ras association-like domain to direct kinase localization. An integral membrane protein, Opy2p can interact with Ste50p, as a component in the Sho1p-Ste11p/Ste50p signaling branch of the high-osmolarity glycerol (HOG) pathway.
Publication Name: Genes & Development
Subject: Biological sciences
ISSN: 0890-9369
Year: 2006
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