Reconstruction of C(sub)3 and C(sub)4 metabolism in Methylobacterium extorquens AM1 using transposon mutagenesis

Article Abstract:

Research describes the development of central heterotrophic and methylotrophic central metabolic architecture in Methylobacterium extorquens AM1 using a mini-transposon5 derivative, ISphoAlhah-Tc. The modified genotypes are selected on the basis of their defective growth on non-C(sub)1 substrates, which identifies the gene interruptions responsible for their phenotypic expression.

Methods, Usage, Microbial metabolism, Microbial genetic engineering, Transposons, Mutagenesis

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A computer investigation of chemically mediated detachment in bacterial biofilms

Article Abstract:

Research has been conducted on three-dimensional bacterial biofilm model. The authors present a description of their study of the model which combines diffusion-reaction equations for chemicals and a cellular automata detachment algorithm used for stimulation of bacterial growth, movement and detachment.

Analysis, Microbial mats, Extraction (Chemistry)

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Promoters and transcripts for genes involved in methanol oxidation in Methylobacterium extorquens AM1

Article Abstract:

Research has been conducted on genes involved in methanol oxidation to formaldehyde in methylotroph Methylobacterium extorquens AM1 consisting of several gene clusters. Results demonstrate that these clusters are transcribed as a single operon.

All Other Basic Organic Chemical Manufacturing, Industrial organic chemicals, not elsewhere classified, Formaldehyde, Methanol, Genetic transcription, Transcription (Genetics), Oxidation-reduction reaction, Oxidation-reduction reactions

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