Genetic manipulation of the pathway for diacetyl metabolism in Lactococcus lactis
Article Abstract:
Lactococcus lactis subsp. cremoris MG1363 mutants lacking the aldB gene produce a large amount of diacetyl. The aldB gene encodes alpha-acetolactate decarboxylase and is deleted by double crossover homologous recombination. The mutants accumulate a large amount of alpha-acetolactate that is oxidatively decarboxylated to diacetyl. The overexpression of the ilvBN genes that encode alpha-acetolactate synthase in the mutants further increases diacetyl production. The diacetyl production in the mutants is comparable to that in L. lactis subsp. lactis biovar diacetylactis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Alternative lactose catabolic pathway in lactococcus lactis IL1403
Article Abstract:
The diversity of the Lactococcus lactis subsp. lactis IL1403 beta-galactosidase phenotype-negative selection on solid media containing cellobiose or lactose and X-Gal was presented and several genes essential for lactose assimilation were identified. The analysis revealed that although phospho-beta-glucosidase, a gene essential for lactose assimilation is homologous to a putative phospho-beta-glucosidase, it also exhibits phospho-beta-glucosidase activity and is the major enzyme in Lactococcus lactis IL1403 in lactose hydrolysis.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2005
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