Reverse transcription-PCR analysis of the regulation of the manganese peroxidase gene family

Article Abstract:

Reverse transcription-polymerase chain reaction was used to investigate manganese peroxidase (MnP) gene expression in the lignin-degrading fungus Phanerochaete chrysosporium. Findings showed that the mnp1 and mnp2 genes from the fungus were regulated by Mn in nitrogen-limited cultures. In contrast, the mnp3 gen did not appear to be significantly regulated by Mn. The mnp2 transcript predominated in stationary cultures, the mnp1 transcript in agitated cultures, while the mnp3 transcript peaks on day three then returns to basal levels.

Usage, Genetic aspects, Manganese, Manganese (Metal), Fungi, Polymerase chain reaction, Metalloenzymes, Peroxidase

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1,4-benzoquinone reductase from Phanerochaete chrysosporium: cDNA cloning and regulation of expression

Article Abstract:

Research was conducted to isolate and characterize a cDNA clone encoding the white rot basidiomycete Phanerochaete chrysosporium quinone reductase and to examine the regulation of quinone reductase synthesis. Results based on immunoblot analysis reveal that induction leads to an increase in the amount of quinone reductase protein. A Northern blot analysis showed evidence that the synthesis regulation takes place at the level of the quinone reductase mRNA.

Genetic regulation, Cloning, Messenger RNA, Circular DNA, Quinone, Quinones, Amino acids

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The green fluorescent protein gene functions as a reporter of gene expression in Phanerochaete chrysosporium

Article Abstract:

The green fluorescent protein from Aequorea victoria can be used to report gene expression in Phanerochaete chrysosporium, especially when an intron is inserted into the enhanced gfp gene. This reporter system can be used to study the regulation of genes in P. chrysosporium that code for lignin-degrading enzymes.

Measurement, Gene expression, Microbial genetics

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