Rhizobium leguminosarum nodulation gene (nod) expression is lowered by an allele-specific mutation in the dicarboxylate transport gene dctB
Article Abstract:
Insertion of a nodC-phoA reporter plasmid with nodD into a symbiotic plasmid-absent Rhizobium leguminosarum strain that is chemically mutagenized helps delineate the host genes that affect nod gene expression in R. leguminosarum. The mutant resulting from insertion of the symbiotic plasmid pRL1Jl after curing the nodC-phoA plasmid exhibits normal nodule formation with 80% symbiotic nitrogen fixation. Introduction of the nodC-phoA allele on PRL1Jl leads to low level nod gene activation. Genetic analysis reveals the presence of IS 50 in the mutant regulatory gene dctB, leading to reduced nod gene expression is not affected by other mutations in dctB, indicating that the effect is allele-specific.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1995
User Contributions:
Comment about this article or add new information about this topic:
Identification of symbiosis-specific c-type cytochromes and a putative oxidase in bacteroids of Rhizobium leguminosarum biovar viciae
Article Abstract:
Previous research has been successful in characterizing the haem proteins in Rhizobium leguminosarum bacteroids. In an extension of past studies, the spectra of two symbiosis-specific c-type cytochromes and covalently bound haem proteins in R. leguminosarum biovar viciae free-living cells and nitrogen-fixing bacteroids are characterised using a dual wavelength spectrometer. Results indicate that the synthesis of the two cytochromes have been repressed. The presence of a ligand-reactive haem protein that is a candidate for the high-affinity symbiosis-specific terminal oxidase in the bacteroids is also detected.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
Analysis of a Rhizobium leguminosarum gene encoding a protein homologous to glutathione S-tranferases
Article Abstract:
The biochemical characteristics of the gst-like Rhizobium leguminosarum gene belonging to the glutathione S-transferase (GST) gene family were analyzed in Escherchia coli. Analysis of Rhizobium leguminasarum gene overexpressed in Escherichia coli indicated the presence of a 21990 Da polypeptide with a 203 amino acid sequence that was encoded by the GST gene. Furthermore, the GST gene exhibited an antigenic domain that was different from mammalian GST genes.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1997
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Haplotype structure and population genetic inferences from nucleotide-sequence variation in human lipoprotein lipase
- Abstracts: Genotype/phenotype analysis of a photoreceptor-specific ATP-binding cassette transporter gene, ABCR (ital), in Stargardt disease
- Abstracts: Ralstonia solanacearum pectin methylesterase is required for growth on methylated pectin but not for bacterial wilt virulence
- Abstracts: Influence of carbohydrate starvation and arginine on culturability and amino acid utilization of Lactococcus lactis subsp. lactis
- Abstracts: The strength of selection on ultraconserved elements in the human genome