Ribulose-1,5-bisphosphate carboxylase/oxygenase gene expression and diversity of Lake Erie planktonic microorganisms

Article Abstract:

Ribulose-1,5-bisphosphate carboxylase/oxygenase (RubisCO) large-subunit genes (rbcL) mRNA are different at different levels of water, indicating a stratification of active CO2-fixing organisms in Lake Erie. Amino acid sequence analysis indicates that five out of six clones for surface water samples represent green algae. Six out of nine rbcL clones from 10-m-deep water samples represent genes from chromophytic and rhodophytic lineages. The amount of rbcL gene expression for the samples is determined from the amount of gene expression per gene dose.

Natural history, Identification and classification, Microorganisms, Gene expression, Phytoplankton, Lake Erie, Clone cells

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Overproduction of recombinant ribulose 1,5-bisphosphate carboxylase/oxygenase from Synechococcus sp. strain PCC6301 in glucose-controlled high-cell-density fermentations by Escherichia coli K-12

Article Abstract:

Glucose control and high cell density fermentation produces heterologous, cyanobacterial recombinant hexadecameric (L8S8) protein, and ribulose 1,5-bisphosphate carboxylase/oxygenase (RubisCO) in Escherichia coli. Optimization of culture and plasmid stability conditions produces about 1 gram of soluble, active recombinant RubisCO per litre. Lactose-induced high cell density fermentation of E. coli K-12 also gives recombinant RubisCO.

Usage, Observations, Escherichia coli, Fermentation, Ribulosebiphosphate carboxylase

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Rhodobacter capsulatus genes encoding form I ribulose-1,5-bisphosphate carboxylase/oxygenase (cbbLS) and neighbouring genes were acquired by a horizontal gene transfer

Article Abstract:

The non-sulfur purple bacterium Rhodobacter capsulatus is examined to identify the nucleotide sequence of its form I ribulose-1,5-bisphosphate carboxylase/oxygenase genes. Results show that the identified amino acid sequence is not closely homologous to the sequences of related bacteria. Subsequent analysis of gene organization and phylogeny indicates that the questioned genes were derived through a horizontal gene transfer.

Bacterial genetics, Bacteria, Photosynthetic, Photosynthetic bacteria

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