Role of reciprocal exchange, one-ended invasion crossover and single-strand annealing on inverted and direct repeat recombination in yeast: different requirements for the RAD1, RAD10, and RAD52 genes

Article Abstract:

Examination of deletions and inversions in Saccharomyces cerevisiae using novel DNA substrates based on the same repeat sequence reveals the involvement of reciprocal exchange, one-ended invasion crossover and single-strand annealing (SSA) in the recombination events. Both deletions and inversions are induced by crossing over, while only deletions are stimulated by SSA. The influence of different factors in regulating the contribution of crossing over and SSA to deletion is discussed. RAD52 gene exerts no influence on SSA, while one-ended invasion crossover is dependent on RAD52. The functions of RAD1 and RAD10 genes in the recombination events are analyzed.

Analysis, Chromosome deletion, Inversion (Genetics)

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A Saccharomyces cerevisiae RAD52 allelle expressing a C-terminal truncaion protein: activities and intragenic complementation of missense mutations

Article Abstract:

The nonsense allele rad52-327 of yeast RAD 52 gene was compared to two missense alleles, rad52-1 and rad52-2, and a deletion allele in terms of activity. The rad52-1 and deletion mutants had been found to have defects in DNA repair and recombination. The rad52-327 and rad52-2 mutants have partial or complete capabilities and had performed similarly in most test of repair and recombination and were found to be complementary for repair and sporulation. The difference between rad52-327 and rad52-2 lie in their ability to sporulate where failure was noted with the latter and weak activity with low spore viablity with the former.

Evaluation, Complementation (Genetics)

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Plasmid recombination in a rad52 mutant of Saccharomyces cerevisiae

Article Abstract:

The pathway of DNA repair and heteroallelic recombination mediated by RAD52 was elucidated by using recombination reporter plasmids containing his3 heteroalleles in either direct repeat or inverted repeat orientations. Results using a rad52 mutant strain showed that rad52 decreased the rate of inverted repeat recombination without affecting the distribution of products formed. The rad52 mutation also decreased direct repeat recombination but not as drastically as for inverted repeats. Additionaly, more cross-over events were detected after direct repeat recombination, which also generated larger recombination products.

Plasmids

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