Saccharomyces cerevisiae expressing bacterial polyhydroxybutyrate synthase produces poly-3-hydroxybutyrate
Article Abstract:
Saccharomyces cerevisiae cells expressing the Alcaligenes eutrophus polyhydroxybutyrate (PHB) synthase gene produce PHB by a pathway identical to that in A. eutrophus. The synthesis is catalyzed by the bacterial PHB synthase, and the yeast beta-oxidation protein and cytoplasmic acetoacetyl-CoA thiolase. The fatty acid synthesis pathway is not involved in PHB synthesis. Ethanol formation takes place along with the PHB synthesis. The cells accumulate almost 0.5% of their dry weight as PHB during the stationary growth phase.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 1996
User Contributions:
Comment about this article or add new information about this topic:
The gene cluster for chloramphenicol biosynthesis in Streptomyces venezuelae ISP5230 includes novel shikimate pathway homologues and a monomodular nonribosomal peptide synthetase gene
Article Abstract:
Research has been conducted on the Streptomyces venezuelae ISP5230. The cloning of the chloramphenicol biosynthesis cluster directed to the regions of the S. venezuelae ISP5230 chromosome flanking pabAB has been carried out and investigated.
Publication Name: Microbiology
Subject: Biological sciences
ISSN: 1350-0872
Year: 2001
User Contributions:
Comment about this article or add new information about this topic:
- Abstracts: Cloning of the Alcaligenes latus polyhydroxyalkanoate biosynthesis genes and use of these genes for enhanced production of poly(3-hydroxybutyrate) in Escherichia coli
- Abstracts: Linear correlation between bacterial overexpression of recombinant peptides and cell light scatter. Method for rapid purification of class IIa bacteriocins and comparison of their activities
- Abstracts: Defects in Saccharomyces cerevisiae protein phosphatase type I activate the spindle/kinetochore checkpoint. TSC1 and TSC2 tumor suppressors antagonize insulin signaling in cell growth
- Abstracts: Avian hairy gene expression identifies a molecular clock linked to vertebrate segmentation and somitogenesis. FGF signaling controls somite boundary position and regulates segmentation clock control of spatiotemporal Hox gene activation
- Abstracts: Escherichia coli is unable to produce pyrroloquinoline quinone. Antigen 43, the major phase-variable protein of the Eshcericha coli outer membrane, can exist as a family of proteins encoded by multiple alleles