Seasonal distributions of dominant 16S rRNA-defined populations in a hot spring microbial mat examined by denaturing gradient gel electrophoresis
Article Abstract:
Denaturing gradient gel electrophoresis analysis of PCR-amplified 16S rRNA gene segments were employed to investigate the distributions of bacterial populations within a hot spring microbial mat. A new cyanobacterial 16S rRNA sequence type was observed at temperatures from 63 to 75 degrees C. A new green nonsulfur bacterium-like sequence type was also found at temperatures from 53 to 62 degrees C. Genetically unique though closely related cyanobacterial and green nonsulfur bacterium-like populations were successively distributed along the thermal gradient of the Octopus Spring effluent channel. At least two cyanobacterial populations were found at each site.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1997
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Denaturing gradient gel electrophoresis profiles of 16S rRNA-defined populations inhabiting a hot spring microbial mat community
Article Abstract:
Denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene segments is an efficient method for determining the distribution of different microbes in the hot Octopus springs of the Yellowstone National Park. The DGGE method rapidly tests the amplified sequences. The sequencing of DGGE bands allows the simultaneous detection of many microbial mat populations. The results compare well with the oligonucleotide probe analysis. The cyanobacteria, which are phenotypically similar, adapt to different temperatures in the hot springs.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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Enrichment culture and microscopy conceal diverse thermophilic Synechoccus populations in a single hot spring microbial mat habitat
Article Abstract:
The behavior of type A,B,J and Synechococcus lividus (S. lividus) Y-7c-s populations during enrichment culture in a hot spring microbial mat habitat has been investigated. 16S rRNA sequences of S. lividus isolates from low-and high-dilution enrichment cultures were compared. Results indicated a connection between biodiversity in the S. lividus population and guild structure.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1996
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