Identification of functionally related genes that stimulate early meiotic gene expression in yeast

Article Abstract:

The IME1 gene, stimulated by the MCK1 gene, initiates meiosis and spore formation in Saccharomyces cerevisiae by activating sporulation-specific genes, one of which is the IME2. To identify the regulators of IME1 expression, screening for mutants of failed IME2 expression was adopted through the use of rme1 mutation to assay IME2 expression and ime2-lacZ fusion gene to screen for mutants with diminished IME2 expression. Twelve Regulator of IME2 (RIM) genes were isolated and were found to encode related products that are similar to MCK1 for IME1 expression stimulation.

Evaluation, Identification and classification, Gene expression, Genetic code

---

Genetic evidence for transcriptional activation by the yeast IME1 gene product

Article Abstract:

The role of IME1 in the transcriptional activation of IME2 expression was discussed. In this study, a reporter gene with upstream lexA binding sites was activated in vivo by a fusion protein containing IME1 and lexA DNA-binding domain. The activity of the fusion protein was found to be similar to that of the natural IME1. Immunofluorescent analysis showed the concentration of IMEE1-lac z fusion protein in the nucleus. The results indicate that IME1 provides a transcriptional activation domain at the IME2 5' regulatory region.

Genetic aspects, Genetic transcription, Transcription (Genetics), Meiosis

---

Similar abstracts:

• Abstracts: Selection for early meiotic mutants in yeast. Stage-specific effects of X-irradiation on yeast meiosis. A RAD9-dependent checkpoint blocks meiosis of cdc13 yeast cells

This website is not affiliated with document authors or copyright owners. This page is provided for informational purposes only. Unintentional errors are possible.