Selection for early meiotic mutants in yeast
Article Abstract:
IME1 is a meiotic activator in Saccharomyces cerevisiae which is expressed at higher levels under starvation conditions. Experiments conducted on haploid S. cerevisiae cells under starvation conditions showed that IME1 expression under these conditions is toxic, due to meiotic induction. The toxic effect of IME1 is enhanced by mutation in rad52. A search for IME1 suppressors led to the isolation of two genes, RIM11 and RIM16. The products of both of these genes are required for IME1 to activate other genes involved in meiosis. Mapping and characterization of RIM11 showed that it is a newly mapped gene, which is located near CIN4 on chromosome XIII.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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Stage-specific effects of X-irradiation on yeast meiosis
Article Abstract:
A study was conducted on the effect of chromosomal lesions on the meiotic process by exposure of yeast cells to X-irradiation at various stages during sporulation. Results revealed that RAD9 cell exposure to X-irradiation arrested sporulation before the cells could undergo premeiotic DNA replication. A checkpoint function in meiosis that is dependent on RAD9 and which is different from cdc13 dysfunction-caused G2 arrest was also found. This function, however, was not found when the lesions are induced much later, suggesting that the lesions can be repaired by acting as intermediates during recombination.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1993
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A RAD9-dependent checkpoint blocks meiosis of cdc13 yeast cells
Article Abstract:
CDC13 andRAD9 are proteins which are involved in mitotic cell cycle arrest of Saccharomyces cerevisiae. The roles of these proteins in meiotic arrest was examined by mutational analysis of their respective genes, cdc13 and rad9. Mutation in cdc13 resulted in arrest of the meiotic division at a stage prior to spindle formation but after DNA replication. This block can be suppressed bymutation in rad9, indicating a role for RAD9 in meiotic arrest. However, RAD9 in itself is not essential for ongoing meiosis. The possible roles of other meiotic checkpoints in yeast are discussed.
Publication Name: Genetics
Subject: Biological sciences
ISSN: 0016-6731
Year: 1992
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