Sequence analysis and molecular characterization of the Lactococcus lactis temperate bacteriophage BK5-T
Article Abstract:
Researchers complete the genetic analysis of bacteriophage BK5-T of Lactococcus lactis by describing the remaining 21-kbp region of the genome. A description of approximately 19 kbp was published in 1995.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 2001
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Sequence analysis of the Lactococcus lactis temperate bacteriophage BK5-T and demonstration that the phage DNA has cohesive ends
Article Abstract:
The Lactococcus lactis temperate bacteriophage BK5-T genome possesses cohesive ends. Analysis of the sequence of 18,935 base pairs indicate the phage DNA contains 32 open reading frames (ORFs) which are longer than 60 codons. The DNA is not terminally redundant. Nucleotide sequence analysis by restriction cleavage identifies discrepancies in the BK5-T genomic map reported previously. These revelations may lead to the revision of the relationship between BK5-T and other Lactococcus phages.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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Spontaneous deletion mutants of the Lactococcus lactis temperate bacteriophage BK5-T and localization of the BK5-T attP site
Article Abstract:
The sequences of the host attachment regions attP and attB are conserved in many Lactococcus (L.) lactis phages and L. lactis strains, and deletion mutants of bacteriophage BK5-T are formed by growing the phage on strains of L. lactis subspecies cremoris H2. The amino acid sequence of the BK5-T integrase gene is identified. Phage and host attachment sites are identified by characterizing a mutant phage.
Publication Name: Applied and Environmental Microbiology
Subject: Biological sciences
ISSN: 0099-2240
Year: 1995
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